Statistical removal of background signals from high-throughput 1H NMR line-broadening ligand-affinity screens
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چکیده
منابع مشابه
Statistical removal of background signals from high-throughput (1)H NMR line-broadening ligand-affinity screens.
NMR ligand-affinity screens are vital to drug discovery, are routinely used to screen fragment-based libraries, and used to verify chemical leads from high-throughput assays and virtual screens. NMR ligand-affinity screens are also a highly informative first step towards identifying functional epitopes of unknown proteins, as well as elucidating the biochemical functions of protein-ligand inter...
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NMR ligand affinity screening is a powerful technique that is routinely used in drug discovery or functional genomics to directly detect protein-ligand binding events. Binding events can be identified by monitoring differences in the 1D (1)H NMR spectrum of a compound with and without protein. Although a single NMR spectrum can be collected within a short period (2-10 min per sample), one-by-on...
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ژورنال
عنوان ژورنال: Journal of Biomolecular NMR
سال: 2015
ISSN: 0925-2738,1573-5001
DOI: 10.1007/s10858-015-9962-3